Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称:人肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
货号:GEH0109
价格: ¥2400.00 ¥1650.00 ¥10200.00
产品详情
产品应用 | ELISA |
别称 | TNF;DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F;TNF alpha |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人TNF-α抗体包被于酶标板上,实验时样品(或标准品)中的人TNF-α会与包被抗体结合。后依次加入生物素化的抗人TNF-α抗体和辣根过氧化物酶标记的亲和素,抗人TNF-α抗体与结合在包被抗体上的人TNF-α结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TNF-α浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TNF-α的浓度。 |
反应类型 | Sandwich-ELISA |
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法、ELISA、夹心法 |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人TNF-α,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
产品结果图
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