Human TIMP-2(Tissue Inhibitors of Metalloproteinase 2) ELISA Kit

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Human TIMP-2(Tissue Inhibitors of Metalloproteinase 2) ELISA Kit-1

中文名称：人基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫吸附测定试剂盒

货号：GEH1453

规格：

96 T 48 T 96 T × 5

产品详情

产品应用	ELISA
别称	TIMP2;CSC-21K;DDC8;CSC-21Ktissue inhibitor of metalloproteinase 2;metalloproteinase inhibitor 2;TIMP metallopeptidase inhibitor 2;Tissue inhibitor of metalloproteinases 2
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗人TIMP-2抗体包被于酶标板上，实验时样品（或标准品）中的人TIMP-2会与包被抗体结合。后依次加入生物素化的抗人TIMP-2抗体和辣根过氧化物酶标记的亲和素，抗人TIMP-2抗体与结合在包被抗体上的人TIMP-2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TIMP-2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TIMP-2的浓度。
反应类型	Sandwich-ELISA
反应时间	3 h 30 min
反应性	Human
检测方法	比色法、ELISA、夹心法
检测范围	0.16-10 ng/mL
灵敏度	0.1 ng/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的人TIMP-2，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月

产品结果图

Human TIMP-2(Tissue Inhibitors of Metalloproteinase 2) ELISA Kit-产品结果图-1

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