英文名称:HT-29
货号:GCL-0118
价格: ¥1600.00 ¥1600.00
产品详情
产品信息
物种 | Human |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:McCoy's 5A[GPM150710]+10% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | HT-29细胞是由J·Fogh在1964年用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的;近来,已建株的培养细胞用含相同血清McCoy's 5A培液培养。HT-29细胞在裸鼠中致瘤,也能在类固醇处理的地鼠中致瘤。HT-29细胞合成IgA、CEA、蛋白绑定TGF-β的分泌成分和粘液素等。 |
年龄(性别) | 44Y |
组织来源 | 结直肠腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
基因表达情况 | secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin, myc+; ras+; myb+; fos+; sis+; p53+; abl -; ros -; src -, Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5, HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). |
致瘤性 | Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters. |
受体表达情况 | human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A |
保藏机构 | ATCC; HTB-38 BCRJ; 0111 CCLV; CCLV-RIE 0960 DSMZ; ACC-299 ECACC; 91072201 |
相关文献
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期刊:Bioengineered
DOI:10.1080/21655979.2021.1996505
影响因子:4.900
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期刊:Cytometry Part A
影响因子:3.700
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Strictosamide alleviates the inflammation in an acute ulcerative colitis (UC) model(2021)
期刊:Journal Of Physiology And Biochemistry
DOI:10.1007/s13105-021-00796-y
影响因子:3.400
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Osthole induces apoptosis of the HT-29 cells via endoplasmic reticulum stress and autophagy(2021)
期刊:Oncology Letters
影响因子:2.900
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期刊:Oncology Letters
影响因子:2.900
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期刊:Pathology Research And Practice
影响因子:2.800
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期刊:PeerJ
影响因子:2.700
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期刊:Experimental and Therapeutic Medicine
影响因子:2.700
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SARS-CoV-2 induced intestinal responses with a biomimetic human gut-on-chip(2020)
期刊:Science Bulletin
DOI:10.1016/j.scib.2020.11.015
影响因子:18.900
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期刊:Journal Of Agricultural And Food Chemistry
影响因子:6.100