英文名称:Hep 3B2.1-7
货号:GCL-0102
价格: ¥1600.00 ¥1600.00
产品详情
产品信息
物种 | Human |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:无血清非程序冻存液(GPB180438)/通用血清型程序冻存液(GPB180436) 温度:液氮 |
培养方案A(默认) |
生长培养基:MEM(含NEAA)[GPM150410]+10% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
推荐传代比例 | 1:2-1:3 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | Hep 3B2.1-7细胞是来自8岁男性黑人的组织。Hep 3B2.1-7细胞的染色体模式数目为60,在裸鼠中能致瘤。Hep 3B2.1-7细胞整合了完整的乙型肝炎病毒基因组,需在2级生物安全防护下操作。 |
年龄(性别) | 8Y |
组织来源 | 肝细胞癌,肝 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肝癌细胞 |
生物安全等级 | BSL-2 |
基因表达情况 | alpha-fetoprotein (AFP, alpha fetoprotein); hepatitis B surface antigen (HBsAg); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; ,alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen complement (C3, C4), C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); ,alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein) |
致瘤性 | Yes, in nude mice. |
保藏机构 | ATCC; HB-8064 BCRC; 60434 BCRJ; 0357 DSMZ; ACC-93 ECACC; 86062703 |
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