英文名称:Hela
货号:GCL-0101
价格: ¥1400.00 ¥1400.00
产品详情
产品信息
物种 | Human |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 |
冻存液:55% 基础培养基+40% FBS+5% DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:MEM(含NEAA)[GPM150410]+10% FBS[163210]+1% P/S[GPB180120] 培养条件: 温度:37℃ |
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
背景描述 | Hela细胞是第一个来自人组织和连续培养维持非整倍体上皮样细胞系。Hela细胞是由G·D·Gey等在1951年从31岁女性黑人的宫颈癌建立。经原始组织切片样品的重新观察,Jones等将其诊断为腺癌。已知该细胞系含有人乳头状瘤病毒18序列,需在2级生物安全防护台操作。Hela细胞角蛋白阳性,p53表达量较低,但表达正常水平的Prb(视网膜母细胞瘤抑制因子)。 |
年龄(性别) | 31Y |
组织来源 | 宫颈腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 宫颈癌细胞 |
生物安全等级 | BSL-2 |
基因表达情况 | Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway. The cells are positive for keratin by immunoperoxidase staining. |
保藏机构 | ATCC; CCL-2 BCRC; 60005 BCRJ; 0100 DSMZ; ACC-57 ECACC; 08011102 ECACC; 93021013 |
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