英文名称：Panc 08.13
货号：GCL-0797
价格： ￥1800.00 ￥1800.00
规格：
1×10^6Cells/T25
1×10^6Cells/Vial×2Vials
产品详情
产品信息
| 物种 | Human |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 |
冻存液：55% 基础培养基+40% FBS+5% DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基：RPMI-1640[GPM150110]+15% FBS[163210]+10 μg/mL Insulin[GPB180432]+1% P/S[GPB180120] 培养条件： 温度：37℃ |
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
参考资料（来源文献）
| 背景描述 | Panc 08.13 is an epithelial cell line that was isolated in 1995 from the pancreas of a White, 85-year-old, male patient with adenocarcinoma. This cell line was deposited by EM Jaffee and can be used in cancer research.The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. The cells have a reported plating efficiency of 40%. |
| 年龄（性别） | 85Y |
| 组织来源 | 胰腺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 胰腺癌细胞 |
| 生物安全等级 | BSL-1 |
| 抗原表达情况 | MHC class I +; MHC class II - |
| 基因表达情况 | cytokeratin 7; cytokeratin 18 |
| 致瘤性 | Yes; Yes, forms tumors in nude or SCID mice. |
| 保藏机构 | ATCC; CRL-2551 |
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