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  • HCC70-1
HCC70-1

英文名称:HCC70

货号:GCL-0822

价格: ¥2500.00 ¥2500.00

规格:
1×10^6Cells/T25 1×10^6Cells/Vial×2Vials
产品详情

产品信息

物种 Human
生长特性 贴壁细胞
细胞形态 上皮细胞样
冻存条件 冻存液:55% 基础培养基+40% FBS+5% DMSO
温度:液氮
培养方案A(默认) 生长培养基:RPMI-1640[GPM150110]+10% FBS[163210]+1% P/S[GPB180120]
培养条件: 温度:37℃
推荐传代比例 1:4-1:6
推荐换液频率 2-3次/周
注意事项
  1. 该细胞培养过程中有黑点,换液后仍会出现是正常现象,建议一周换2-3次液。
参考资料(来源文献)
背景描述 HCC70 is an epithelial cell line isolated from a primary ductal carcinoma from a 49-year-old, Black female in 1992, and the cells took 44 months to establish. HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. The cells are poorly differentiated. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
年龄(性别) 49Y
组织来源 乳腺
细胞类型 肿瘤细胞
肿瘤类型 乳腺导管癌
生物安全等级 BSL-1
基因表达情况 epithelial glycoprotein 2 (EGP2); cytokeratin 19
保藏机构 ATCC; CRL-2315
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