AF/LE Purified Anti-Mouse CD150 Antibody[TC15-12F12.2]

C57/BL6 Mouse splenocytes were stained with 0.2 μg AF/LE Purified Anti-Mouse CD150 Antibody[TC15-12F12.2] (Right) and 0.2 μg Rat Ig2a, κ Isotype Control (Left), followed by Elab Fluor^® 647-conjugated Goat Anti-Rat IgG Secondary Antibody.

货号：GF11770

规格：

100 μg 1 mg

产品详情

Background

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Alternate Names

SLAM family member;SLAMF;CD 150;CD150;CD150 antigen;Signaling lymphocytic activation molecule;SLAM family member 1;Slam;Slamf1; IPO-3

Swissprot

Q9QUM4

Immunogen

Recombinant Mouse CD150 protein

Clone No.

TC15-12F12.2

Host

Rat

Reactivity

Mouse

Isotype

Rat IgG2a, λ

Dilution

FCM 2 μg/mL(0.5×10⁶-1×10⁶ cells)

Conjugate

None (AF/LE)

Concentration

≥ 1 mg/mL

Purification Method

>98%, Protein A/G purified

Applications

FCM

Clonality

Monoclonal

Storage Buffer

Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Storage

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Expiration Date

12个月

Shipping

Ice bag